N-glycosylation in Sulfolobus acidocaldarius

Almost all extracellular proteins of archaea are modified by either N- or O-glycosylation or both. We have set out to analyze the N-glycosylation pathway in S. acidocaldarius and used the S-layer protein as a substrate protein. We identified a number of enzymes that play an important role in the N-glycosylation pathway in S. acidocaldarius.

Papers:

Meyer BH, Shams-Eldin H, Albers SV.(2017) AglH, a thermophilic UDP-N-acetylglucosamine-1-phosphate:dolichyl phosphate GlcNAc-1-phosphotransferase initiating protein N-glycosylation pathway in Sulfolobus acidocaldarius, is capable of complementing the eukaryal Alg7.

Extremophiles. 2017 Jan;21(1):121-134. doi: 10.1007/s00792-016-0890-2.

Meyer BH, Albers SV. (2014) AglB, catalyzing the oligosaccharyl transferase step of the archaeal N-glycosylation process, is essential in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius.

Microbiologyopen. 2014 Aug;3(4):531-43. doi: 10.1002/mbo3.185.

Meyer BH, Peyfoon E, Dietrich C, Hitchen P, Panico M, Morris HR, Dell A, Albers SV. (2013) Agl16, a thermophilic glycosyltransferase mediating the last step of N-Glycan biosynthesis in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius.

J Bacteriol. 2013 May;195(10):2177-86. doi: 10.1128/JB.00035-13.

Meyer BH, Zolghadr B, Peyfoon E, Pabst M, Panico M, Morris HR, Haslam SM, Messner P, Schäffer C, Dell A, Albers SV. (2011) Sulfoquinovose synthase - an important enzyme in the N-glycosylation pathway of Sulfolobus acidocaldarius.

Mol Microbiol. 2011 Dec;82(5):1150-63. doi: 10.1111/j.1365-2958.2011.07875.x.

Peyfoon E, Meyer B, Hitchen PG, Panico M, Morris HR, Haslam SM, Albers SV, Dell A. (2010) The S-layer glycoprotein of the crenarchaeote Sulfolobus acidocaldarius is glycosylated at multiple sites with chitobiose-linked N-glycans.

Archaea. 2010 Sep 29;2010. pii: 754101. doi: 10.1155/2010/754101.